项目名称：新型多孔智能聚合物材料在生物分离与操纵中的应用

项目来源：国家自然科学基金青年科学基金项目

项目编号：21104061

项目金额：26万元

生物分离是现代生物技术应用的必要前提，开发具有特定生物分子识别功能的智能膜材料可以很好得解决目前生物分离手段中的一些突出问题，同时具有清洁、高效、特异性好、费效比低等优点，具有广阔的应用前景。智能聚合物材料，特别是生物分子响应性材料是目前材料科学研究的一个热门方向，包括申请者课题组在内的国内国际多个研究组已经发展出了多种能对外界刺激（如光电热磁）具有良好响应性的智能界面材料。我们拟将生物分子响应性智能材料与多孔膜基底相结合，开发出一系列膜通道孔径可调的多孔智能聚合物膜材料。同时选取三类DNA 作为生物分离研究的模型对象，系统评估上述智能聚合物膜对目标DNA 的分离效果；同步观测聚合物链在纳米通道中，响应生物分子对象时所产生的伸缩行为，以及其对生物分子的操纵行为，探索可能的分离作用机制。本项目开发研究的智能膜材料将在生物分离与纯化、生物芯片、微流体器件、药物释放等多方面具有良好的应用前景。

Title of funded project：Novel nanoporous smart polymeric materials and their applications in bioseparation and manipulation.

Funding agency：National Natural Science Foundation of China

Grant number：21104061

Total funding amount：CNY 260,000

Bio-separation is the essential precondition of the applications of modern biotechnology. In order to solve some outstanding problems in the bio-separation, it is emergent to develop some smart nanoporous membrane materials which could recognize specific biomolecules. These materials will find wide applications due to their obvious advantages such as clean, high efficiency and specificity, excellent cost performance. Smart polymeric materials, especially the biomolecules responsive materials have become one of popular directions in the material sciences. Various smart interfacial materials which could response to the external stimulus, such as heat, pH, light, electric and magnetic fields have been developed by many domestic and international research groups, also including our own contributions. In this project, we will combine the bioresponisve smart materials with the nanoporous alumina oxide substrates to develop a series of smart nano-channels membrane materials, in which the pore sizes of nanochannels could be adjusted conveniently. In the further step, three classes of DNAs will be chosen as the model targets of bio-separations, the separation effects of the channel materials will be evaluated systematically. The inner channel behaviours of polymeric chains changing from contractive state to relaxed state in response to the biomolecules will also be investigated synchronously. Finally the possible mechanism of bioseparations will be explored in detail. The smart membrane materials developed in this project will have wide application perspectives in the bio-separation and purification, controllable drug release, bio-chips, microfluid devices and other bio-related areas.