生命科学和生物技术产业的巨大进步提高了对生物分离的要求。目前的色谱和相关分离技术已经被提升到很高的程度，它们对蛋白质组学，药物和食品工业中的复杂样品提供了有效的分离能力。然而，在更复杂的应用场景中仍然非常需要新的突破，例如更高的选择性，更可控的分离模式，更低的成本和更温和的操作条件。作为新型分离介质，可以可逆地改变其结构和性质，以响应外部刺激的智能材料，为升级当前分离技术提供了理想的解决方案。在这篇综述中，深入地讨论了面向生物分离应用的智能材料的最新进展，总结了智能材料在通过控制模式改善分离性能方面的优势。同时提出了当前遇到的挑战和可能的解决方案，以有利于智能生物分离材料的设计和前瞻性的应用。

Smart bio-separation materials

Minmin Li, Yuting Xiong, Guangyan Qing*

Trends in Analytical Chemistry, 2019, 124, 115585.

DOI: 10.1016/j.trac.2019.06.035

Tremendous advances of life science and biotechnology industry have raised the higher requirement for bio-separation. Current chromatographic and related separation techniques have been refined to such a degree that they present efficient separation capacity towards complex samples in proteomics, pharmaceuticals and food industry. Nevertheless, new breakthroughs, like higher selectivity, more controllable separation mode, lower cost, and milder operation conditions, are still highly desirable in more complicated application scenarios. Smart materials that could reversibly change their structures and properties responding to external stimuli as novel separation media provide an ideal solution to upgrade current separation techniques. In this review, recent advances on smart materials in bio-separation applications are intensively discussed, with an emphasize on the superiority of smart materials in improving the separation performance by a controlled mode. And current challenges encountered and conceivable solutions are also presented to benefit prospective development in design and applications of smart bio-separation materials.