糖基化是哺乳动物细胞中最常见和最重要的蛋白质翻译后修饰类型之一。为了解码蛋白质糖基化，从复杂的生物样品中富集糖肽或分离糖链至关重要的。亲水相互作用液相色谱（HILIC）在亲水物质的分离和糖肽富集方面具有固有的优点。常规HILIC显示出与亲水性溶质的无偏向性的结合，导致糖肽的富集选择性不理想。近三年来，开发的许多新型HILIC材料，包括组氨酸，二肽，麦芽糖和基于酰胺的材料，显示出对唾液酸化糖肽的特异性结合能力，或对中性糖肽的更强的保留。因此，HILIC已经发展成为同时收集糖基化位点和糖链结构信息的高效方法。在本综述中，我们总结了HILIC材料应用于糖肽富集和糖链分离的最新突破，并对HILIC未来的发展趋势进行了展望。

Recent advances in hydrophilic interaction liquid interaction chromatography materials for glycopeptide enrichment and glycan separation

Guangyan Qing, Jingyu Yan, Xiangnan He, Xiuling Li**, Xinmiao Liang*

Trends in Analytical Chemistry, 2019, 124, 115570.

DOI: 10.1016/j.trac.2019.06.020

Glycosylation is one of the most common and important types of protein post-translational modifications in mammalian cells. To decode the protein glycosylation, it is highly important to enrich glycopeptides or separate glycans from complex biosamples. Hydrophilic interaction liquid interaction chromatography (HILIC) has inherent advantages in the separation of hydrophilic substances and glycopeptide enrichment. Conventional HILIC display unbiased binding with the hydrophilic solutes, leading to unsatisfactory enrichment selectivity for glycopeptides. In recent three years, many novel HILIC materials, including histidine, dipeptide, maltose, and amide-based ones, were developed and displayed either specific binding capacities toward sialylated glycopeptides, or substantially stronger retention toward neutral glycopeptides. Therefore, HILIC has grown up to be a high-efficiency method for collecting both glycosylation sites and glycan structural information simultaneously. In the review, recent breakthrough of HILIC materials applied to glycopeptide enrichment and glycan separation is summarized, and the future development tendency of the HILIC is suggested.